The effectiveness of CAR-T cells and non-transduced T-cells in the lysis of SKBR3 tumour cells was measured by the CellTiter 96 Cell Proliferation Assay (MTS) (Promega, Madison, WI, USA) and Fluorescein Isothiocyanate (FITC) Annexin V Apoptosis Detection Kit (Becton Dickinson (BD), Franklin Lakes, NJ, USA). The gene discussed is ANXA5; the disease is neoplasm.