A cut-off value of 0.090 for CRY-1 gene expression in CD19+ B cells of CLL patients was previously determined [6] and considered the best cut-off for segregating patients with mutated and unmutated IgHV genes (sensitivity: 95%; specificity: 92%; area under the curve: 0.963). The gene discussed is CRY1; the disease is B-cell chronic lymphocytic leukemia.