CYCS and ischemia: After addition of exogenous cytochrome c during state 3 respiration, respiratory rate (V3 + cyt c) with glutamate + malate increased by 9% and 12% and with succinate by 32% and 93% (p < 0.05) after 20 min and 40 min of ischemia, respectively, as compared to the control group (Figures 2(a) and 2(b)), indicating that ischemia induced damage of mitochondrial outer membrane, which increased with the duration of ischemia.