In the TGF-β/MAPK signaling pathway, TGF interacted with ERK1/2, JNK, and p38 MAPK, playing an active role in myocardial fibrosis.FTI276 could suppress ERK1/2 phosphorylation, and kallikrein, OMT, and STZ could inhibit ERK1/2 and JNK/p38 MAPK phosphorylation to decrease VR. This evidence concerns the gene KLK4 and Myocardial fibrosis.