Eight cytokines (IL-8, IL-10, sCD40L, TNF-α, MCP-1, MIP-1α, RANTES and LIGHT) and 19 biochemical indices (ALT, AST, GGT, ALB, TP, TB, DB, TBA, UN, Cr, UA, ADA, ApoAI, ApoC, SOD, SF, CHE, ALP and LDH) were analyzed in aGVHD patients and non-GVHD control subjects at each time point. The gene discussed is TNF; the disease is graft versus host disease.