Furthermore, KYSE450, KYSE510, and KYSE30 esophageal cancer cells treated with the compound exhibited substantial up-regulation of apoptosis-associated proteins, including cleaved-PARP, cleaved caspase 3 and pro-apoptotic Bax whereas anti-apoptotic Bcl-2 expression was decreased (Figure 2B). This evidence concerns the gene BCL2 and esophageal cancer.