Therefore, to investigate the role of TLR2 or TLR4 in BCG-dependent AMPs release in tumor cells, we generated stable TLR2 or TLR4 knockdown lines from T24 cells and confirmed the reduced expression of TLR2 or TLR4 by Western blotting (Figure 4A, upper panel). The gene discussed is TLR4; the disease is neoplasm.