The results showed that the high-invasive colon cancer cell line HCT-8 had higher expression levels of Twist1 compared with the low-invasive HT-29 cells and normal colon cell line NCM460 and vincristine treatment upregulated Twist1 expression only in the invasive colon cancer cells (Figure 1A–1B). This evidence concerns the gene TWIST1 and colonic neoplasm.