Flow cytometry was used to determine the programmed cell death (PCD)-inducing activity of H2-18 and GDC-0941 in BT-474, SKBR-3, HCC-1954 and HCC-1419 cell lines by using FITC Annexin V Apoptosis Detection Kit I. The results showed that in these four cell lines, all of the treatments, H2-18, GDC-0941, and H2-18 plus GDC-0941, could effectively increase cell death (Figure 5). This evidence concerns the gene ANXA5 and hepatocellular carcinoma.