There is also increased risk of cancer-related death with increasing age (≥ 55 vs. < 55, P = 0.035), pT stage (T3 vs. T2, P = 0.030, T4 vs. T3, P = 0.035), pN stage (N2 vs. N1, P = 0.023), liver metastasis (P = 0.004), pathologic TNM stage (III vs. II, P = 0.033; IV vs. III, P = 0.009), and cfDNA methylation level of CBS promoter in plasma of patients (LPM group vs. HPM group, P = 0.009). The gene discussed is CBS; the disease is metastasis.