Comparison of the influence of cytokines or cytokine combination on the PD-1 and CTLA-4 mRNA levels in PBMC from normal adults and cancer patients revealed that the PD-1 and CTLA-4 expression increased in most cancer patients prior to cytokine treatment, and was hardly modulated after the treatment (Figure 3B), indicating the intrinsic inhibition of the immune function by tumors. The gene discussed is CTLA4; the disease is cancer.