In order to clarify apoptosis or necrosis, we conducted Annexin V-FITC/PI assay and the results determined by flow cytometry showed that percent of apoptosis cells of HNSCC was increased apparently after treatment with CUR (Figure 2A–2F), indicating that CUR has the ability to induce apoptosis of HNSCC. Here, ANXA5 is linked to head and neck squamous cell carcinoma.