To further confirm that the Wnt/β-catenin pathway may be associated with the role of LGR5 in cancer stemness, CHIR-99021 (CT99021), an inhibitor of GSK-3β that suppresses β-catenin degradation, was used to activate the Wnt/β-catenin pathway in LGR5-knockdown SiHa and HeLa cells. The gene discussed is GSK3B; the disease is cancer.