To investigate the role of LGR5 on the self-renewal capacity of cervical cancer cells, which is one of the most important characteristics of CSCs, LGR5-overexpressing cells (SiHa-LGR5 and HeLa-LGR5) and LGR5-knockdown cells (SiHa-shLGR5 and HeLa-shLGR5), as well as control cells, were cultured in serum-free medium under conditions that are optimal for growing tumorspheres. This evidence concerns the gene LGR5 and cervical carcinoma.