Given the known tumor suppressor role of both TSC1 and PTEN (Inoki et al., 2005), we used the more restricted MpzCre mouse line (Feltri et al., 1999) to generate suitable single or double mutants, referred to as MpzCre:Tsc1KO, MpzCre:PtenKO, and MpzCre:Tsc1KO:PtenKO. Here, PTEN is linked to neoplasm.