Our cytotoxicity data demonstrated that after 9 h, untreated PAO1 infection appeared to compromise hCF monolayer integrity; therefore, IL-1β cytokine was measured at this time-point in supernatants of PAO1-infected (MOI = 1, 10, 100) and un-infected hCFs (MOI = 0) monolayers following addition of the different antibiotics after 3 h of infection (Figure 6). This evidence concerns the gene IL1B and infection.