To test whether ECHS1 senses nutrients via acetylation, we first confirmed that the acetylation levels of ectopically expressed ECHS1 from HEK293T and other cultured cancer cells, probed by a pan-acetyl lysine antibody (α-AcK)26, were each increased by glucose, FAs, and amino acids (Fig. 2a–c, Supplementary Fig. 3). Here, ECHS1 is linked to cancer.