To confirm the role of neuronal O-GlcNAcylation on cerebral ischemia-reperfusion injury, we developed a neuron-specific brain OGT knockout (KO) mouse model, in which the OGT gene was floxed by two loxP sites and the CRE expression was induced by tamoxifen administration and controlled by the CaMK-IIα promoter. This evidence concerns the gene CAMK2A and brain ischemia.