To confirm the role of neuronal O-GlcNAcylation on cerebral ischemia-reperfusion injury, we developed a neuron-specific brain OGT knockout (KO) mouse model, in which the OGT gene was floxed by two loxP sites and the CRE expression was induced by tamoxifen administration and controlled by the CaMK-IIα promoter. The gene discussed is CAMK2A; the disease is Cerebral ischemia.