In the combined WES and standard testing cohorts the five most frequently tested genes were sequencing of ANO5, CAPN3, FKRP, LMNA and sequencing of TTN exons 293 and 308, which are mutation hotspots for Hereditary Myopathy with Early Respiratory Failure, a relatively frequent diagnosis in the North of England [29]. Here, ANO5 is linked to respiratory failure.