Here, we used spinal cord homogenates prepared from a total of four SOD1-FALS (A4V, D90A, G93S, I113T), three SALS, one healthy control, and three non-ALS controls (AD, MSA), to show that only homogenates prepared from SOD1-FALS can effectively trigger the aggregation of chimeric SOD1-GFP protein with G37R, G85R or G93A mutations in the SOD1 moiety. This evidence concerns the gene SOD1 and amyotrophic lateral sclerosis.