Previous studies showed that mutant SOD1 inclusions prepared in vitro can seed insoluble aggregation of cytosolic soluble mutant SOD1 [7], consistent with our current findings that mutant SOD1 seeds from FALS-SOD1 homogenates lead to aggregation of the reporter proteins; 2) misfolded wild-type SOD1 found in sporadic ALS without obvious ubiquitinated SOD1 inclusions. This evidence concerns the gene SOD1 and amyotrophic lateral sclerosis.