IRF6 and melanoma: The association between the methylation status of the 5' IRF6 CGI (S1 Fig) and transcription of IRF6 as analyzed by RT-MSP and RT-PCR, respectively (Fig 1), for each of the melanoma cell lines (RPMI-7951, A375, Hs294T, SK-MEL-28, SK-MEL-2, SK-MEL-5, SH-4 and G-361) and normal cultured melanocytes (MC1 and MC2) was as follows.