We next infected caspase-1/caspase-11 double deficient mice with N67C, and again did not detect any differences in host mortality, parasitemia, or body weight between KO and WT mice (Supplementary Fig. S6c–e), suggesting that inflammasome-mediated cell death or pyroptosis might not play a significant role in disease severity and pathology in N67C infection. This evidence concerns the gene CASP1 and parasitic infectious disease.