In the present study, the protein levels of MDA, 4-HNE, aconitase-2, and 8-OHdG were upregulated in corneal tissue with fungal infection, which indicated that oxidative stress occurred in fungal keratitis and caused the corneal tissue, and especially corneal epithelial cells, to undergo oxidative injury, including via lipid peroxidation, intracellular protein modification, and DNA oxidative damage. The gene discussed is ACO2; the disease is fungal keratitis.