Mutations in the second most highly mutated putative driver in breast cancer, DHS chr6:28948439-28951450 (Fig. 3a), were associated in Filter 3 with the overexpression of a known cancer gene (TRIM27), a member of the tripartite motif (TRIM) family of E3 ubiquitin-protein ligases that is involved in several cancer types through interactions with RARα, RB, p300, ERBB2, RET and JUN34. The gene discussed is EP300; the disease is breast cancer.