TGFA and hepatocellular carcinoma: Reverse transcription quantitative (RT-q)PCR analysis showed that HUVECs promoted gene expression related to cell proliferation, including cyclins (Supplementary Fig. S3e), and HCC-associated oncogenes such as IGF2, cMET, RHOA, TCF4, TNFSF10, and TGFA (Supplementary Fig. S3f), which was verified by conventional RT-PCR (Supplementary Fig. S3g).