To determine whether wsp-1, the C. elegans orthologs of genes that interact with TNK2 (lst-1, dhs-7, and nck-1), or C. elegans genes that interact with WSP-1 (mig-13, mig-2, and nck-1) affect Orsay virus infection, we performed RNAi knockdown and evaluated Orsay virus replication. This evidence concerns the gene LST1 and viral infectious disease.