GST proteins linked to the extreme C-terminus of POM121C containing the α-helix motif but not the FG repeat motifs or simply to the α-helix motif failed to inhibit HIV-1 infection or to interact with KPNB1 (Fig 6F, 6G and 6H), suggesting that the α-helix motif alone is not sufficient for HIV-1 blockade or binding to KPNB1. The gene discussed is KPNB1; the disease is HIV-1 infection.