APC and malignant colon neoplasm: 2016) and anti-colon cancer activity (Volate et al. 2005). Rutin has been shown to target different modulators of Wnt signalling intracellularly (Perk et al. 2014), this action perhaps can cause negative modulation of APC gene (adenomatous polyposis coli). APC is one of the genes that directly involves in carcinogenesis of colon cancer, thus preventing the APC gene forming its complex transcriptional factor might inhibit the early stage of colon cancer by M. calabura leaves.