To further analyze FOXO3-function in high-stage NB cells, we used an ectopically expressed 4OHT-inducible, PKB-phosphorylation-independent FOXO3(A3)ER fusion-protein and infected high-stage NB1, NB3, NB4, NB8 and NB15 cells.14, 28 The expression and translocation of ectopic FOXO3 after 4OHT-treatment was verified by immunoblot (Figures 1b and d and Supplementary Figure S1b). This evidence concerns the gene FOXO3 and neuroblastoma.