We demonstrated before that in NB cells FOXO3 is frequently PKB-phosphorylated and that especially in high-stage-derived NB cells FOXO3 partially localizes to the nucleus despite highly active PKB (Figures 1a and c, Supplementary Figures S1a and c).14, 27 In these cells, oxidative stress and DNA-damage further increase the amount of nuclear FOXO3, suggesting that the cell-stress-dependent regulation of FOXO3 is still functional (Figure 1c). This evidence concerns the gene FOXO3 and neuroblastoma.