Western blot analysis of tumor homogenates derived from these preparations revealed a reduction in AKT phosphorylation levels following MAG-EPA treatment when compared with control mice (Figure 5c), which translated into significant reductions of 80% and 71% in P-AKTser/AKT and P-AKTthr/AKT ratios, respectively, after the normalization of identical immunoblot membrane areas (Figure 5d). This evidence concerns the gene AKT1 and neoplasm.