However, EPF supplementation in HFD-induced obesity mice for eight weeks markedly ameliorated insulin resistance conditions, as indicated by lower serum glucose and insulin levels at the end of the experiment compared to the HFD group, which is thought to stimulate the pancreatic insulin secretion from the β cells of the islets of langerhans and aldose reductase enzyme inhibitory activity [20]. Here, AKR1B1 is linked to obesity due to melanocortin 4 receptor deficiency.