To further evaluate whether HNF1A‐AS1 in sera can be a potential diagnostic biomarker for patients with osteosarcoma, a ROC curve using serum ALP and HNF1A‐AS1 was established for 30 osteosarcoma patients (by using the serum collected at 1 hr before surgery) and 21 healthy individuals. This evidence concerns the gene HNF1A and osteosarcoma.