The correlation of HNF1A‐AS1 overexpression with a higher tumour burden including presence of distance metastasis and advanced TNM stage in our study, suggesting that HNF1A‐AS1 may accelerate tumour progression in osteosarcoma mainly via stimulating malignant phenotype of osteosarcoma cells, which was supported by the results of knockdown of HNF1A‐AS1 in MG63 and U2OS cells. Here, HNF1A is linked to osteosarcoma.