To see whether glucose or insulin resistance played a role in osteocalcin expression in MG-63 cells during osteogenic cell differentiation, we performed time-course experiments in which MG-63 cells, either untreated or pre-treated with palmitate to induce in vitro insulin resistance, were incubated with low (2 mM) or high (25 mM) glucose for 6–14 days. Here, BGLAP is linked to Insulin resistance.