Moreover, inactivation of NLRP3 inflammasome by MCC950 could not reduce NLRP3, pro-Caspase-1 protein expression level in vitro, but we observed decreased expression of NLRP3 and pro-Caspase-1 in the MCC950 treated Tgfbr1/Pten 2cKO SCCHN mice model compared to control group. The gene discussed is CASP1; the disease is head and neck squamous cell carcinoma.