To determine whether NLRP3 inflamasome was associated with human HNSCC, we examined their expression by immunohistochemistry and found that NLRP3, Caspase-1, ASC, IL-18 were highly expressed in the cytoplasm of cancer cells, and the expression of them was negative or low in the normal mucosa (Fig. 1a). This evidence concerns the gene CASP1 and head and neck squamous cell carcinoma.