Our results revealed intense staining of NLRP3, ASC, Caspase-1, and IL-18 expression in Tgfbr1/Pten 2cKO SCCHN mice tumor lysates compared with control wild type mice tongues (Fig. 2a), these results indicated that the NLRP3 inflammasome was activated, and very high level of NLRP3 inflammasome was found in Tgfbr1/Pten 2cKO SCCHN mouse model. Here, CASP1 is linked to head and neck squamous cell carcinoma.