First, although astrocytes were found to be the dominant cell type expressing SUR1-TRPM4 in chronic EAE and MS, definitive characterization of the role of SUR1-TRPM4 in astrocytes will require the study of conditional knockout animals with astrocyte-specific deletion of Abcc8 or Trpm4. Second, although we found that circulating leukocyte counts were not affected by glibenclamide administered to normal animals, these findings do not completely exclude a potential contribution of non-CNS effects of the drug in EAE. Here, TRPM4 is linked to myeloid sarcoma.