Moreover we used an established dorsal skinfold window chamber model [25] to examine the in vivo in real-time tumor sprouting angiogenesis under two-photon microscope in Apln-CreERT:mT/mG mice, we observed green eGFP sprouting angiogenesis in the tumor vasculature, but not in the normal skinfold tissue (Fig. 5a-c). This evidence concerns the gene APLN and neoplasm.