In the human hepatoma Huh7 cell line, upregulation of hepcidin expression through the canonical BMP pathway increased H3K9ac at the HAMP promoter, while suppression of HAMP mRNA expression using a BMP receptor inhibitor (LDN193189) reduced enrichment for this mark (Fig. 3e; Supplementary Fig. 2c). Here, HAMP is linked to hepatocellular carcinoma.