To further assess the expression of CXorf48 in LSCs, we sorted the CD34+CD38− cells from the CML cell line KU812, since LSCs of myeloid leukemia are considered to be enriched in this fraction.16, 17 The results of quantitative PCR showed that CD34+38− cells isolated from the CML cells, KU812, expressed higher level of CXorf48 gene compared to CD34− cells (Figure 2a). This evidence concerns the gene CD38 and chronic myelogenous leukemia, BCR-ABL1 positive.