The expression of total PI3K, Akt, AMPK protein in each group was equal, while p-PI3K, p-Akt and p-AMPK were significantly lower in the AKI and CO + AKI groups compared with the control group (p < 0.01) (Fig. 6b), and was markedly up-regulated in the CUR + AKI group compared with the AKI and CO + AKI groups (p < 0.01). The gene discussed is AKT1; the disease is acute kidney injury.