To further test if p.Gly132Ala and p.Tyr920ThrfsX40 are pathogenic, we turned to a zebrafish model where MO knockdown of tmem67 leads to various morphological defects characteristic of ciliopathy, such as hydrocephalus, pronephric cysts, curved trunk and notochord defect30, 31. The gene discussed is TMEM67; the disease is Hydrocephalus.