At mRNA level, treatment with 60 μM 10058-F4 for 72 h led to a modest upregulation of TH but had little or no effect on SCG2, TRKA and NOTCH1 expression, indicating that MYCN inhibition promotes differentiation of neuroblastoma PDX cells to a limited extent (Fig. 3g). This evidence concerns the gene NTRK1 and neuroblastoma.