When the general discriminant analysis technique was used to assess the predictive abilities of combinations of serum and salivary biomarkers for TB disease, a 5-marker biosignature comprising of serum IL-6, MIP-1β, VEGF and saliva G-CSF and MIP-1α, diagnosed TB disease in the training sample set (n = 54; n = 27 TB cases and n = 27 ORD), with a sensitivity of 81.5% (22/27) and specificity of 100% (27/27), and with a sensitivity of 50% (6/12) and specificity of 75% (9/12) in the test sample set (n = 24, n = 12 TB cases and n = 12 ORD). Here, CSF3 is linked to tuberculosis.