NFKB1 and HIV-1 infection: Notably, the results were not biased by multiple rounds of infection as treatment with the protease inhibitor Darunavir did not significantly affect induction of NF-κB activation upon HIV-1 infection or NF-κB inhibition by SIVolc Vpr, although it efficiently blocked de novo generation of infectious HIV-1 particles (S7D–S7F Fig).