When C35 protein was interfered by siRNA1 and siRNA2 for 48 h or 72h, the viability of BT474 breast ductal carcinoma cells decreased compared with the control group (92.0±0.6% and 94.5±1.3% versus 100±1.2%; p < 0.05, Fig 2) or (83.1±3.1% and 88.6±2.3% versus 96.8±0.9%, S1 Fig). This evidence concerns the gene MIEN1 and breast ductal adenocarcinoma.