FMO5 and Familial prostate cancer: To re-examine ROS, we used the free radical scavengers Trolox and Butylated Hydoxyanisole and the NADPH Oxidase inhibitor Diphenyleneiodonium Chloride, and, and just as with NAC (see Fig 5A), these anti-oxidants were unable to rescue Peptide B-8R-induced cytotoxicity and the latter two actually slightly cytotoxic to prostate cancer cells (S3 Fig), verifying that elevated ROS was not responsible the peptide’s cytotoxicity.