To re-examine ROS, we used the free radical scavengers Trolox and Butylated Hydoxyanisole and the NADPH Oxidase inhibitor Diphenyleneiodonium Chloride, and, and just as with NAC (see Fig 5A), these anti-oxidants were unable to rescue Peptide B-8R-induced cytotoxicity and the latter two actually slightly cytotoxic to prostate cancer cells (S3 Fig), verifying that elevated ROS was not responsible the peptide’s cytotoxicity. This evidence concerns the gene FMO5 and prostate carcinoma.