Furthermore, the methylation-inactivated Wnt5a-L mRNA isoform can be reactivated by treatment with a demethylation agent, and knockdown of expression of the Wnt5a-S mRNA isoform can enhance reactivation of Wnt5a-L isoform-mediated downregulation of β-catenin expression, eventually weakening tumor progression of CRC. Here, WNT5A is linked to colorectal carcinoma.