In order to determine whether the putative ERE sequence motifs that we studied in the lymphoblastoid cell lines (LCLs) would also bind to ER in breast cancer cell lines in the presence of E2, we repeated the ChIP assays in the presence of vehicle and E2 using two ER + breast cancer cell lines—ZR-75-1 and MCF-7. The gene discussed is ESR1; the disease is breast cancer.