Taken together, our results may represent a remarkable breakthrough to unveil sALS, supporting a primary role of t-SNARE protein expression as possible cause of intracellular calcium elevation and glutamate excitotoxicity12 and suggesting SNAP25 and related SNARE proteins as candidate novel indicators and signatures of motor neuron death to be monitored in ALS patients. Here, SNAP25 is linked to amyotrophic lateral sclerosis.