Biochemical analysis found that ANIT exposure dramatically increased serum aspartate transferase (AST) and alanine transferase (ALT) (P < 0.001), as well as serum alkaline phosphatase (ALP) (P < 0.001) that is an intrahepatic cholestasis marker (Fig. 1a), indicating a severe cholestasis induced by ANIT. The gene discussed is GPT; the disease is intrahepatic cholestasis.