We found that RetSat expression correlates with liver steatosis in humans and that its hepatic depletion lowers liver triglycerides (TGs) and improves metabolic parameters in dietary obese mice, at least in part, by interfering with the activity of the cellular hexose-phosphate sensor carbohydrate response element-binding protein (ChREBP)13. Here, RETSAT is linked to Hepatic steatosis.