The shortened ORF3 with a deletion of 3 nt in A40, D40, and E40 strains; the early-terminated ORF3 with a 134 nt deletion in B40 and D40 strains; and the shortened Nsp2 with a deletion of 330 nt in B40 strains, were judged not to be involved in the infection characteristics of PEDV strain 85-7 (Figures 3, 5I and J). Here, ASZ1 is linked to infection.